General Notes
This product (Catalog No. CABT-BL517) is a rabbit-derived IgG polyclonal antibody raised against human C11ORF73, targeting the amino acid region 21–120 of the protein. This antibody exhibits cross-reactivity with C11ORF73 from rat, mouse, chicken, dog, pig, cow, rabbit, and sheep. It has been affinity purified by protein A. This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress.
CABT-BL517 is suitable for use in Western blot (WB) (1:500–1:2,000) application.
CABT-BL517 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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