C. difficile Toxin A (DAGH048)

Nature
Native
Tag/Conjugate
Unconjugated
Procedure
None
Purity
>95% pure by SDS-PAGE
Format
Lyophilised
Concentration
0.2 - 0.5 mg/ml
Size
50μg, 100μg, 500μg
Buffer
Contents when reconstituted in 250μl sterile distillcd water will contain: Toxin A at a concentration of 0.2mg/ml in 0.05M Hepes, O. l5M NaCl and 5% sucrose
Preservative
None
Storage
Lyophilised vial: +2°C to +8°C; Reconstituted liquid: +2°C to +8°C. Reconstituted liquid can be frozen at -80°C to -20°C to extend stability to l year; however every freeze/thaw cycle will cause increased aggregation. Avoid multiple freeze/thaw cycles and multiple temperature fluctuations. Mixing: Swirl or pipette gently to mix. Vortexing promotes aggregation.
Stability
Lyophilised vial: 1 year after date of receipt; Reconstituted liquid: 1 month
Antigen Description
Native toxin A purified from C. difficile strain VPI10463 (toxinotype 0). Vcrocytotoxicity assay result: 0.2 ng/ml (lowest point at which rounded cells are observed). Clostridium difficile Toxin A is a highly purified preparation. Developed either for use with clinical diagnostic assays, it serves as a positive control and for determination of assay range or for use by researchers in techniques such as cytotoxicity testing. The product is presented in a choice of pack sizes and is lyophilised for ease of use.
Keywords
Clostridium difficile Toxin A; C. difficile Toxin A; Toxin A; TcdA; The large clostridial cytotoxins; LCT

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