Matched pair antibody available for Butylated Hydroxytoluene [BSA]: Butylated Hydroxytoluene antibody (Catalog # HMABPY107)
Format
Liquid
Concentration
Batch dependent - please inquire should you have specific requirements.
Buffer
0.01M pH7.4 PBS
Preservative
None
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Keywords
Butylated hydroxytoluene;BHT
Citations
Publication ()
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Background
Butylated hydroxytoluene (BHT), alternatively called dibutylhydroxytoluene, is an organic compound that exhibits a strong affinity for lipids. BHT is a substituted derivative of phenol. It has a wide range of applications in various industries, including flavors, fragrances, biochemical reagents, chemical raw materials, biochemical, antioxidants, inorganic salts, feed additives, feed storage additives, bulk drugs, and food additives. It acts as a phenolic antioxidant, protecting against lipid peroxidation and demonstrating toxic effects mediated by oxidative metabolism through a compound called electrophilic quinone methyl ether. However, it is important to note that the metabolites of BHT, particularly 6-tert-butyl-2-[2'-(2'-hydroxymethyl)-propyl]-4-methylphenol, have been associated with potential lung damage in mice and the promotion of tumor growth. Butylated Hydroxytoluene [BSA] refers to a synthetic antigen that is created by combining BHT with bovine serum albumin (BSA). The purpose of developing Butylated Hydroxytoluene [BSA] is to create specific and targeted reagents for use in diagnostic assays such as LFIA (Lateral Flow Immunoassay) and ELISA (Enzyme-Linked Immunosorbent Assay). These assays are commonly used in medical, veterinary, and environmental fields to detect and quantify the presence of specific substances or analytes. By conjugating BHT with BSA, the resulting complex can serve as an antigen, triggering an immune response when introduced into a biological system. This immune response can be harnessed in diagnostic assays to detect the presence of BHT or related compounds in samples.
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References
Understanding the chemistry behind the antioxidant activities of butylated hydroxytoluene (BHT): A review
Hindered phenols find a wide variety of applications across many different industry sectors. Butylated hydroxytoluene (BHT) is a most commonly used antioxidant recognized as safe for use in foods containing fats, pharmaceuticals, petroleum products, rubber and oil industries. In the past two decades, there has been growing interest in finding novel antioxidants to meet the requirements of these industries. To accelerate the antioxidant discovery process, researchers have designed and synthesized a series of BHT derivatives targeting to improve its antioxidant properties to be having a wide range of antioxidant activities markedly enhanced radical scavenging ability and other physical properties. Accordingly, some structure–activity relationships and rational design strategies for antioxidants based on BHT structure have been suggested and applied in practice. We have identified 14 very sensitive parameters, which may play a major role on the antioxidant performance of BHT. In this review, we attempt to summarize the current knowledge on this topic, which is of significance in selecting and designing novel antioxidants using a well-known antioxidant BHT as a building-block molecule. Our strategy involved investigation on understanding the chemistry behind the antioxidant activities of BHT, whether through hydrogen or electron transfer mechanism to enable promising anti-oxidant candidates to be synthesized.
Butylated hydroxytoluene (BHT) induction of pulmonary inflammation: a role in tumor promotion
Experimental Lung Research
Authors: Bauer A K, Dwyer-Nield L D, Keil K, et al.
Chronic pulmonary inflammatory diseases predispose towards lung cancer by unknown mechanisms. Butylated hydroxytoluene (BHT) administration to mice causes lung injury and a subsequent inflammatory response, and, when administered chronically to certain inbred strains following carcinogen treatment, increases lung tumor multiplicity. We hypothesize that inflammation promotes lung tumor growth in this model system and have begun to examine this hypothesis by assessing inflammatory parameters in inbred strains that vary in their susceptibility to promotion. Positive correlations were found between susceptibilities to tumor promotion and BHT induction of alveolar macrophage and lymphocyte infiltration into alveolar airspaces, and increased vascular permeability (P <. 03, P <. 04, and P <. 005, respectively).The amounts of pulmonary cyclooxygenase (COX)-1 and COX-2 did not strongly correlate with promotion. Because persistent elevation of macrophage content is the hallmark of a chronic inflammatory response, the alveolar macrophage population was depleted by adding chlorine to the drinking water prior to carcinogenesis. This treatment reduced lung tumor multiplicity following 2-stage carcinogenesis (P <. 05).These correlations between inflammatory and tumorigenic responses to BHT, along with decreased tumorigenesis after macrophage depletion, are consistent with a role of inflammation in promotion. Inflammatory mediators may provide targets for early diagnosis and chemoprevention.
COA
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