Bisphenol A ELISA Kit

Sample

urine, plasma or serum, cells, tissues

Species Reactivity

All Animals; Human

Detection Method

cELISA

Intended Use

This kit can be used for the determination of BPA in urine, serum, plasma, cells, and tissues following proper isolation and purification. Instructions are provided as to the proper isolation and purification in the following pages. The free BPA level in urine or cell culture media can be measured using the BPA ELISA, without ethyl acetate extraction after 4-fold dilution of the sample.

Contents of Kit

1. BPA ELISA Plate, Solid 96-well plate coated with anti-BPA antibody in each well
2. BPA Standard (2 μL), Stock standard at a concentration of 1 mg/mL
3. BPA-HRP Conjugates (12 μL), 1000× concentrated solution
4. Sample Dilution Buffer (25 mL), 10× solution of Tris-buffered saline with preservatives
5. HRP Buffer (15 mL), 1× solution of Tris-buffered saline with preservatives
6. Wash Buffer Solution (25 mL), 10× solution of Tris-buffered saline with detergents and preservatives
7. TMB Substrate (24 mL), A solution of TMB (tetra methyl benzadine)

Storage

All components are stored below -20°C and should not be re-frozen and thawed more than necessary. This kit will obtain optimal results if all of the components are stored at the proper temperature prior to use. Items should be stored at the designated temperatures upon receipt of this kit.

Detection Range

10-100,000 pg/mL

Sensitivity

10 pg/mL

General Description

BPA is a phenolic environmental estrogen which disrupts endocrine activity. In human, a BPA glucuronide was a primary metabolite of BPA. In a recent study, the age group with highest BPA exposure was 6-11 years old with a mean total (free⁺ glucuronidated) BPA level of 4.33 ng/g of creatinine. Urinary BPA levels were correlated with cardiovascular diseases and diabetes. A recent study revealed that a 12-ounce serving of canned soup for 5 days increased urinary BPA level 12-fold due to BP A-containing epoxy resin lining of the cans.

Standard Curve

The data shown here is an example of typical results obtained using the BPA ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time.
B_L wells = 0.052
B_O wells = 2.870