Intended Use
The Human BZLF1/EBV Zebra gene ELISA kit provides materials for the qualitative and semiquantitative determination of to EBV BZLF1 antigen in human serum, plasma, tissue homogenate and other body fluid.
This assay is intended for research use only.
Contents of Kit
1. Microtiterwells, 12 × 8 (break apart) strips, 96 wells; Wells coated with Epstein-Barr viral BZLF1 antibody.
(incl. 1 strip holder and 1 cover foil)
2. Sample Dilute: 1 vial, 100 mL, ready to use, colored yellow, pH 7.2 ± 0.2.
3. Balance Buffer: 1 vial, 6.5 mL, ready to use, colored yellow.
4. Pos.Control: 1 vial, 1.0 mL, ready to use; colored yellow, red cap.
5. Neg.Control: 1 vial, 2.0 mL, ready to use; colored yellow, yellow cap.
6. Cut-off Control: 1 vial, 2.0 mL, ready to use; colored yellow, black cap.
7. Enzyme Conjugate: 1 vial, 20 mL, ready to use, colored red, antibody to BZLF1 conjugated to horseradish peroxidase.
8. Substrate Solution: 1 vial, 14 mL, ready to use, Tetramethylbenzidine (TMB).
9. Stop Solution: 1 vial, 14 mL, ready to use, contains 0.2 mol/H2SO4. Avoid contact with the stop solution. It may cause skin irritations and bums.
10. Wash Solution: 1 vial, 30 mL (20× concentrated for 600 mL). pH 6.5 ± 0.1 see "Reagent Preparation" contain non-mercury preservative.
Storage
When stored at 2°C to 8°C unopened reagents will retain reacivity until expiration date. Do not use reagents beyond this date.
Opened reagents must be stored at 2°C to 8°C. Microtiter wells must be stored at 2°C to 8°C. Once the foil bag has been opened, care should be taken to close it tightly again.
Opened kits retain activity for two months if stored as described above.