General Notes
This product (Catalog No. CABT-BL4228) is a rabbit-derived IgG polyclonal antibody raised against human BRD4, targeting the amino acid region 65–160 of the protein. This antibody exhibits cross-reactivity with BRD4 from mouse, rat, chicken, dog, pig, cow, horse, and rabbit. It has been affinity purified by Protein A. The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
CABT-BL4228 is suitable for use in ELISA (1:5,000–1:10,000) application.
CABT-BL4228 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.