Porcine Inhibitor of apoptosis protein Like Protein2 ELISA Kit

Name: Porcine Inhibitor of apoptosis protein Like Protein2 ELISA Kit
SKU: DEIA-BJ2817
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Species Reactivity

Porcine

Detection Method

Competitive ELISA

Intended Use

Porcine Inhibitor of apoptosis protein Like Protein2 ELISA Kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Inhibitor of apoptosis protein Like Protein2. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.

Contents of Kit

1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL

Storage

All components of this kit are stable at 2-8°C until the kit's expiration date.

Detection Range

5-100 ng/mL

Sensitivity

0.1 ng/mL

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Differential expression of BIRC family genes in chronic myeloid leukaemia-BIRC3 and BIRC8 as potential new candidates to identify disease progression

BRITISH JOURNAL OF HAEMATOLOGY

Authors: Glodkowska-Mrowka, Eliza; Solarska, Iwona; Mrowka, Piotr; Bajorek, Katarzyna; Niesiobedzka-Krezel, Joanna; Seferynska, Ilona; Borg, Katarzyna; Stoklosa, Tomasz

Abstract

Neem Leaf Extract Induces Radiosensitization in Human Neuroblastoma Xenograft Through Modulation of Apoptotic Pathway

ANTICANCER RESEARCH

Authors: Veeraraghavan, Jamunarani; Aravindan, Sheeja; Natarajan, Mohan; Awasthi, Vibhudutta; Herman, Terence S.; Aravindan, Natarajan

Abstract

Induction of apoptosis is directly correlated with the biological effectiveness of ionizing radiation (IR). Accordingly, we investigated the efficacy of neem leaf extract (NLE) on IR-associated apoptotic transcriptional modulation and cell death in neuroblastoma (NB). Materials and Methods: NB xenografts exposed to single dose (SDR, 10 Gy) or fractionated (FIR, 2 Gyldx5d) with or without NLE were examined for transcriptional activation of 84 apoptotic pathway genes using quantitative polymerase chain reaction. Apoptosis was measured using TdT nick-end labeling. Results: FIR induced 55 and suppressed 10 genes, while SDR induced 49 and suppressed 5 genes. Of these, 46 and 4 genes were commonly up/down-regulated after FIR and SDR. NLE inhibited IR-induced NAIP, BIRC6, BIRC8, NOL3 and enhanced BAKI, BAX, BCL10, CASP1, CASP10 CARD8 and CRADD. Furthermore, NLE conferred FIR- and SDR-induced cell death. Conclusion: These data imply that NLE may exert radiosensitization by activating pro-apoptotic signaling and negating survival signaling and may thus potentiate radiotherapy in NB.