Broad bean wilt virus-1 (BBWV-1) ELISA Kit

A viral isolate, designated N-1 and obtained from a gentian (Gewiana scabra) plant that exhibited mosaic symptoms, was transmitted mechanically to nine plant species in six families. These plants are known as hosts of fabaviruses. The N-1 isolate was composed of isometric particles 30 run in diameter and included two RNA molecules of approximately 6.0 and 3.6 kb in length. as estimated by agarose gel electrophoresis. The RNAs were encapsidated separately in two of the three types of particle. Each particle contained two distinct proteins with Mr values of 39.3 x 10(3) and 26.6 x 10(3), as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Analysis of complete TlUcleotide sequences of the RNAs suggested that each encoded a single large polyprotein, in which putative functional proteins were arranged in a manner similar to those in Broad bean wilt virus / (BBWV-1) and Broad bean wilt virus 2 (BBWV-2). which are members of the genus Fabavirus (family Comoviridae). Analysis of the deduced amino acid sequences of the proteins indicated that those of isolate N-1 shared 38 to 66% identity with those of BBWV-1 and BBWV-2 but only 16 to 42% identity with those of a comovirus, Cowpea mosaic virus. Phylogenetic analysis. based oil the amino acid sequences of RNA polymerase, placed isolate N-1 in a separate lineage front BBWV-1 and BBWV-2. In indirect-enzyme-linked immunosorbent assay. isolate N-1 exhibited distant serological relationship to BBWV-1. BBWV-2. and Lamium mild mosaic virus. another fabavirus. Our results suggest that N-1 represents a new species of Fabavirus. We propose the name Gentian mosaic virus for this new species.

Studies were conducted to determine the cause of an apparently new disorder of globe artichoke (Cynara scolymus L.) characterised by a localised black necrosis in the receptacle of the flower buds. The effects of single and mixed infections by artichoke latent potyvirus (ALV) and broad bean wilt virus-French artichoke (BBWV-FA) in buds with necrosis of three globe artichoke cultivars were studied. Virus-free plants developed diseased buds irrespective of whether the plants were 1 or 2 years old, but the presence of ALV and/or BBWV-FA significantly increased black spot occurrence on 2-year-old plants. Virus effects were dependent on cultivar, cv. Capitan being less susceptible to virus infection than cvs Camus de Bretagne and Castel. The head size of the globe artichoke was found to be related to the rate of necrosis. with a higher incidence for terminal buds. ALV and BBWV-FA did not cause the disorder but favour its development probably by weakening plants through increased leaf transpiration. The results are discussed in relation to a possible physiological disorder exacerbated by the presence of ALV and/or BBWV-FA.