The test can be used to detect BBTMV in infected herbaceous host plant tissues.
Storage
All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.
Sensitivity
Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:30 -1:90.
General Description
Broad bean true mosaic virus (also called Echtes Ackerbohnemosaik-virus, Vicia virus 1, and Viciavirus varians) is a virus first described in 1953 that affects legumes, commonly found in crops of broad bean in both Europe and Northwest Africa. There are no known vectors, although it has been known to transverse long distances between crops. Infection via seed is common, though the virus is also present in sap. BBTMV occurs mainly in Europe and has been reported as the cause of significant virus disease problems in faba bean production in Great Britain and western and eastern European countries. It has also been reported in virus surveys in north Africa, but generally at low incidence and has been reported from China. BBTMV has not been found in surveys of a number of Middle East countries and is not reported from the United States of America.
Standard Curve
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