The endotoxin content, determined using a kinetic chromagenic LAL assay, is 153.5 EU/mg.
Alternative Names
Bacillus anthracis Edema Factor
Format
Lyophilized power, sealed under vacuum
Preservative
None
Storage
Store at 2-8°C prior to reconstitution.
Reconstitution
Anthrax toxin proteins may be reconstituted in sterile purified water, stored at 2-8°C and used successfully within a few hours. However, over longer periods of time, there may be a decline in the enzymatic activity of EF. If it is necessary to store this material, reconstitute it at a concentration of 1 mg/ml. Reconstitution with 1 mg/ml BSA will enhance stability and recovery. It is further recommended that the solution is aliquoted and frozen at either -20°C or -70°C. Avoid repeated freeze-thaw cycles. After the protein has been reconstituted as described above, glycerol may be added to 50% if a liquid is desired at freezer temperatures.
Citations
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