Avermectin/Ivermectin ELISA Kit

Sample

Beef, pork, mutton, and milk samples

Species Reactivity

Universal

Detection Method

cELISA

Intended Use

This product can be used for qualitative and quantitative detection of avermectin and ivermectin residues in beef, pork, mutton, and milk samples.

Contents of Kit

1. 96‑well microplate ×1 (pre‑coated with conjugated antigen)
2. Standards ×6 vials (1 mL/vial) (1 μg/L = 1 ppb = 1 μg/kg): 0 ppb, 1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
3. High-concentration standard (1 mL/vial): 1 ppm
4. Enzyme‑labeled secondary antibody concentrate (1 mL, red cap)
5. Antibody working solution (10 mL, green cap)
6. Substrate A solution (7 mL, white cap)
7. Substrate B solution (7 mL, red cap)
8. Stop solution (7 mL, yellow cap)
9. 20× Wash buffer concentrate (40 mL, transparent cap)
10. 2× Reconstitution solution concentrate (50 mL, blue cap)

Storage

Storage: 2–8°C.
Shelf life: 12 months.

Performance Characteristics

Accuracy:
Tissue samples: 90% ± 20%
Milk samples: 90% ± 20%

Precision

Intra‑assay and inter-assay coefficients of variation are both <10%.

Detection Limit

Limit of detection (LOD) for tissue (beef/pork/mutton):
Ivermectin: 5 ppb
Avermectin: 2.5 ppb

LOD for milk:
Ivermectin: 3 ppb
Avermectin: 1.5 ppb

Sensitivity

1 ppb

General Description

Avermectins and Ivermectin are semi-synthetic macrolide polycomponent antibiotics produced by fermentation of Streptomyces avermitilis. As a novel broad-spectrum, highly effective, and low-toxicity antibiotic anthelmintic, they show good killing activity against internal and external parasites, especially nematodes and arthropods. However, instrumental methods such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) require complicated sample preparation, tedious operations, and high costs, which limit their widespread use.

This kit is a new-generation drug residue detection product developed using ELISA technology. Compared with instrumental analysis, it offers rapid, simple, accurate, and highly sensitive performance. The total operation time is only 45 minutes, minimizing operational errors and workload.

Standard Curve

Two methods are available: qualitative estimation (Method 1) and quantitative analysis (Method 2). Note that sample absorbance is inversely proportional to the avermectin/ivermectin concentration.
1. Qualitative estimation:
Compare the average absorbance of the sample with the standard values to estimate the concentration range (ppb).
Example standard absorbance values (0 ppb: 2.043; 1 ppb: 1.662; 3 ppb: 1.152; 9 ppb: 0.593; 27 ppb: 0.289; 81 ppb: 0.115).
Sample 1 absorbance 0.701 → range 3–9 ppb (multiply by dilution factor for actual residue concentration).
Sample 2 absorbance 1.302 → range 1–3 ppb (multiply by dilution factor).
2. Quantitative analysis:
(1) Calculate the percentage of absorbance for each standard and sample:
B/B₀ (%) = (Average absorbance of standard or sample / Average absorbance of 0-ppb standard) × 100%
where B = absorbance of standard or sample; B₀ = absorbance of 0-ppb standard.
(2) Prepare a standard curve by plotting the percentage absorbance (ordinate) against the logarithm of the standard concentration (ppb, abscissa). Interpolate the sample concentration from the curve, then multiply by the corresponding dilution factor to obtain the actual residue concentration in the sample.
For large numbers of samples, using the kit' s professional analysis software is recommended for fast and accurate results (contact the supplier for details).
Sample dilution factors:
Beef, pork, mutton: 5-fold
Milk: 3-fold