Apelin C-Terminus ELISA Kit

Name: Apelin C-Terminus ELISA Kit
SKU: DEIA2182
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma

Species Reactivity

Human

Detection Method

Competitive ELISA

Intended Use

Apelin-C Terminus ELISA Kit is designed to target the C-terminus of the 77-aa apelin peptide, thus the active forms of Apelin including Apelin-36 and Apelin 13 can be detected.

Contents of Kit

1. Apelin-C Terminus Microplate
2. Wash Buffer Concentrate (20X)
3. Standard Apelin-C Terminus Peptide
4. Anti-Apelin-C Terminus polyclonal antibody
5. Assay Diluent A
6. Assay Diluent B
7. Biotinylated Apelin-C Terminus peptide,
8. HRP-Streptavidin concentrate
8. 5, 000 X concentrated HRP-conjugated Streptavidin
9. Positive control
10. TMB One-Step Substrate Reagent
11. Stop Solution
12. Assay Diagram
13. User Manual

Storage

Standard, Biotinylated Apelin-C Terminus peptide, and positive control should be stored at -20°C or -80°C. For more detailed information, please download the following document on our website.

Detection Range

0.01-1000 pg

Sensitivity

29.1 pg/mL

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

microRNA-182 Negatively Influences the Neuroprotective Effect of Apelin Against Neuronal Injury in Epilepsy

NEUROPSYCHIATRIC DISEASE AND TREATMENT

Authors: Dong, Han; Dong, Bin; Zhang, Na; Liu, Songyan; Zhao, Huiying

Abstract

Purpose: To explore the neuroprotective effects and mechanisms of Apelin (APLN), and to study the regulation of APLN expression by microRNA (miRNA) in epilepsy. Materials and Methods: In vitro and in vivo epileptic models were established with hippocampal neurons and Wistar rats. Apoptosis of neurons was identified by flow cytometry. Western blotting was used to detect the expression of proteins, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to analyze the expression of miRNA and messenger RNA (mRNA). Bioinformatics software was used to predict target genes of miRNA, which were confirmed by dual-luciferase reporter gene system and functional experiments. Results: Our study demonstrated protective effects of APLN against neuronal death in epilepsy both in vitro and in vivo. The underlying mechanisms involved are inhibiting the expression of metabotropic glutamate receptor 1 (mGluR1), Bax, and caspase-3; promoting the expression of Bc1-2; and increasing phosphorylated-AKT (p-AKT) levels in neurons. For the first time, we found that miR-182 could negatively regulate both transcriptional and translational levels of APLN, and that the up-regulation of miR-182 inhibited the expression of APLN and Bc1-2, and promoted the expression of Bax and caspase-3. Conclusion: APLN could protect the neurons from injury in epilepsy by regulating the expression of apoptosis-associated proteins and mGluR1 and increasing p-AKT levels, which were attenuated by miR-182. Hence, miR-182/APLN may be potential targets for epilepsy control and treatment.

Broad flattened bandwidth wavelength conversion based on difference-frequency generation in aperiodically poled lithium niobate

APOC 2002: ASIA-PACIFIC OPTICAL AND WIRELESS COMMUNICATIONS, OPTIAL FIBER AND PLANAR WAVEGUIDE TECHNOLOGY II

Authors: Song, YJ; Zhan, L; Ye, QH; Xia, YX

Abstract

We propose a general routine, Genetic-Simulated Annealing method (GSA) for the optimal design of quasi-phase matched (QPM) non-uniform grating. Here this method is applied to the design of the flat broadened wavelength conversion bandwidth of 40-mm aperiodically poled lithium niobate (APLN) based on difference-frequency generation (DFG). The flat conversion bandwidth of APLN is broadened from the original 65 nm in a 40-mm periodically poled lithium niobate (PPLN) to the pre-designated 130 nm in a 40-mm APLN, which conversion efficiency is 6.7 dB higher than that of 12.4-mm PPLN under the same conversion bandwidth.