Anti-Yersinia pestis Hybridoma [7C6] (CSC-H0021LN)

This hybridoma produces mAbs (IgG1) against Yersinia pestis

General Information


Immunogen
Y. pestis
Isotype
IgG1
Immunological Donor
Mouse
Clone
7C6
Cell Line Description
The hybridoma produces a mouse monoclonal antibody recognizing Y. pestis surface antigens other than F1, allowing for temperature-independent detection. It can detect Y. pestis at temperatures lower than 37C, detect both F1 and non-F1 antigen fractions, is highly Specific and has no cross reactivity with other enterics
Application Notes
ELISA; WB
Format
Frozen
Growth Properties
Adherent

Culture Method


Complete Growth Medium
DMEM, supplemented with 20% calf serum
Storage
Liquid nitrogen vapor phase
Freezing medium: general freezing media

Target


Introduction
Yersinia pestis, best known as the plague, is a gram-negative rod-shaped bacterium responsible for high mortality epidemics throughout human history. Most available immunosassays for Y. pestis are based on detection of F1, the dominant surface antigen expressed by the pathogen when it is grown at 37C. The advantage of detecting Y. pestis at room temperature, allows contamination testing in water, juice, milk and food.

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