General Notes
This product (Catalog No. DPABH-00706) is a rabbit-derived polyclonal antibody raised against human TNNC1, targeting the amino acid region 1–161 of the protein. This antibody exhibits cross-reactivity with Human TNNC1 from mouse and rat. It has been affinity purified by Protein A. Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.
DPABH-00706 is suitable for use in Western blot (WB) (1:500–1:2,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:50–1:500), IF/ICC (1:50–1:500), flow cytometry (FC) (0.40 μg per 10^6 cells in a 100 μl suspension) applications.
DPABH-00706 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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