Anti-TUBA4A monoclonal antibody, clone EN2B

Antibody Isotype

IgG1

Clone

EN2B

Species Reactivity

Chicken

Immunogen

Full length native protein (purified) of Chicken alpha Tubulin (extracted from brain).

Conjugate

Application Notes

Flow Cyt: Use 1μg for 10⁶ cells. ICC/IF: Use a concentration of 1 μg/ml; IP: Use at an assay dependent dilution; IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent concentration. Electron Microscopy: Use at an assay

Alternative Names

TUBA4A; tubulin, alpha 4A; tubulin alpha-4A chain; alpha-tubulin 4; tubulin, alpha 4; tubulin alpha-4 chain; alpha-tubulin isotype M-alpha-4; M[a]4; Tuba4;

Entrez Gene ID

UniProt ID

Antigen Description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").

Pathway

Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Gap junction, organism-specific biosystem;