General Notes
This product (Catalog No. DPABH-13001) is a rabbit-derived polyclonal antibody raised against human TMEM65, targeting the amino acid region 1–178 of the protein. This antibody exhibits cross-reactivity with Human TMEM65 from mouse and rat. It has been affinity purified by Protein A. TMEM65 (Transmembrane protein 65) is an inner-mitochondrial-membrane protein essential for calcium homeostasis and organelle integrity. By binding to and stabilizing the Na⁺/Ca²⁺ exchanger NCLX, it facilitates mitochondrial Ca²⁺ efflux, thereby regulating oxidative phosphorylation and limiting ROS production. In cardiomyocytes, TMEM65 localizes to intercalated discs where it interacts with connexin-43, ensuring normal electrical conduction and preventing arrhythmia. Loss-of-function mutations cause mitochondrial myopathy with neurodevelopmental delay, whereas its over-expression in gastric cancer activates the YWHAZ-PI3K-Akt-mTOR axis, promoting tumorigenesis. Thus, TMEM65 links mitochondrial quality control to cytoplasmic signaling, positioning it as a potential therapeutic target in metabolic and oncologic diseases.
DPABH-13001 is suitable for use in Western blot (WB) (1:5,000–1:50,000), IHC (1:500–1:2,000), IF/ICC (1:200–1:800) applications.
DPABH-13001 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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