Anti-Scl-70 ELISA Kit

Sample

Serum

Species Reactivity

Universal

Detection Method

ELISA

Intended Use

Anti-Scl-70 ELISA kit measures anti-Scl-70 antibodies in the serum.

Contents of Kit

1. 8×12 96-well plate coated with Scl-70.
2. Anti-human IgG antibody conjugated to HRP.
3. 1× Diluent buffer.
4. 5× Assay wash buffer.
5. Substrate.
6. Stop Solution.

Storage

1. Store all reagents at 2-8°C.
2. All reagents must be brought to room temperature (20-25°C) prior to use.
3. When stored at 2-8°C, the diluted Assay wash buffer is stable until the kit expiration date.

General Description

Antibodies to Scl-70 are a specific immunological marker for scleroderma (or progressive systemic sclerosis, PSS), a systemic autoimmune disease characterized by collagen deposition and connective tissue destruction of the skin, blood vessels and certain internal organs. Studies have shown varying frequencies of Scl-70 antibodies in PSS. This antibody was found in approximately 20% of PSS patients in early studies but 75% in later studies. Scl-70 antibodies are directed against DNA-topoisomerase I which locates in the nucleus. The whole molecule of DNAtopoisomerase is 110 kDa but it is easily degraded by proteases to 100 kDa, 87 kDa and 70 kDa (Scl-70). PSS is classified into two types; diffuse scleroderma and limited scleroderma. Scl-70 antibodies are present specifically in diffuse scleroderma and centromere antibodies are present in limited scleroderma. Rarely, Scl-70 antibodies are found in SLE and MCTD patients.