Anti-STRC (aa 411-541) polyclonal antibody

Background and Objectives Primitive human hematopoietic cells contain higher levels of aldehyde dehydrogenase (ALDH) activity than their terminally differentiating progeny but the particular stages at which ALDH levels change have not been well defined. The objective of this study was to compare ALDH levels among the earliest stages of hematopoietic cell differentiation and to determine whether these could be exploited to obtain improved purity of human cord blood cells with long-term lympho-myeloid repopulating activity in vivo. Design and Methods ALDEFLUOR-stained human cord blood cells displaying different levels of ALDH activity were first analyzed for co-expression of various surface markers. Subsets of these cells were then isolated by multi-parametric flow cytometry and assessed for shortand long-term repopulating activity in sublethally irradiated immunodeficient mice. Results Most short-term myeloid repopulating cells (STRC-M) and all long-term lympho-myeloid repopulating cells (LTRC-ML) stained selectively as ALDH(+). Limiting dilution analysis of the frequencies of both STRC-M and LTRC-ML showed that they were similarly and most highly enriched in the 10% top ALDH(+) cells. Removal of cells expressing CD2, CD3, CD7, CD14, CD16, CD24, CD36, CD38, CD56, CD66b, or glycophorin A from the ALDH' low-density fraction of human cord blood cells with low light side-scattering properties yielded a population containing LTRC-ML at a frequency of 1/360. Interpretation and Conclusion Elevated ALDH activity is a broadly inclusive property of primitive human cord blood cells that, in combination with other markers, allows easy isolation of the stem cell fraction at unprecedented purities.

A new type of steel reinforced concrete (SRC) transfer beam, the steel truss reinforced concrete (STRC) transfer beam, is developed and applied to a 100 m tall building in Guangzhou City to solve the difficulty of two built subways being buried under the building. Experimental studies on mechanical behaviours of STRC composite transfer beams were carried out, and the structural characteristics and economic characters of the transfer beam were introduced. Experimental investigations verify that the STRC transfer beam is of high limit bearing capacity, large rigidity and good ductility. Compared with normal reinforced concrete transfer beam, the limit bearing capacity of the STRC transfer beam can be enhanced 30-40%, and the rigidity can be improved 30-50%. In the STRC transfer beam, the strut-and-tie force transference mode is followed, the loads are mainly transferred by compressive diagonal SRC struts from the loading point and abutments, SRC struts and horizontal ties form a self-balance system. The cost of material, fireproof and construction can be saved; more space for commercial function and convenient construction can be achieved by adopting STRC transfer beams. Copyright (C) 2010 John Wiley & Sons, Ltd.