Anti-SMPD3 (full length) polyclonal antibody

Addition of boron to SmPd3 seems to shift the E(F) towards the Sm2+ level and causes the Kondo effect in the SmPd3B(x) system. In the course of this experiment, it is also found that the sample of LaPd3 prepared by using an arc furnace shows a long-period superstructure, but the single crystal of LaPd3 made in the sealed Mo crucible by the Bridgman method only have the AuCu3-type structure.

Accumulating evidence suggests that chemopreventive effects of some dietary polyphenols in part may be mediated by their ability to influence epigenetic mechanisms in cancer cells. Boswellic acids, derived from the plant Boswellia serrata, have long been used for the treatment of various inflammatory diseases due to their potent anti-inflammatory activities. Recent preclinical studies have also suggested that this compound has anticancer potential against various malignancies. However, the precise molecular mechanisms underlying their anticancer effects remain elusive. Herein, we report that boswellic acids modulate the DNA methylation status of several tumor suppressor genes in colorectal cancer (CRC) cells. We treated RKO, SW48 and SW480 CRC cell lines with the active principle present in boswellic acids, acetyl-keto-beta-boswellic acid (AKBA). Using genome-wide DNA methylation and gene expression microarray analyses, we discovered that AKBA induced a modest genome-wide demethylation that permitted simultaneous re-activation of the corresponding tumor suppressor genes. Quantitative methylation-specific PCR and RT-PCR validated the gene demethylation and re-expression of several putative tumor suppressor genes including SAMD14 and SMPD3. Furthermore, AKBA inhibited DNMT activity in CRC cells. Taken together, these results lend further support to the growing notion that the anticancer effect of boswellic acids in part may be due to its ability to demethylate and reactivate methylation-silenced tumor suppressor genes. These results suggest that boswellic acid might be a promising epigenetic modulator in the chemoprevention and treatment of CRC, and also provide a rationale for future investigations on the usefulness of such botanicals for epigenetic therapy in other human malignancies.