Anti-SMARCA5 monoclonal antibody, clone FQS8784(3)

Antibody Isotype

IgG

Clone

FQS8784(3)

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SNF2H. The exact sequence is proprietary.Database link: O60264

Conjugate

Application Notes

WB: 1/1000 - 1/10000; ICC: 1/250.

Alternative Names

SMARCA5; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; hISWI; hSNF2H; ISWI; sucrose nonfermenting-like 5; s

Entrez Gene ID

UniProt ID

Antigen Description

Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at Tyr-142, and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.

Pathway

Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Deposition of New CENPA-containing Nucleosomes at the Centromere, organism-specific biosystem; Nucleosome assembly, organism-specific biosystem;