Anti-SHH monoclonal antibody (DCABY-3927)

Rat Anti-Mouse SHH monoclonal antibody for WB, IHC, ELISA(Cap), Neut Datasheet

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Specifications


Host Species
Rat
Antibody Isotype
IgG2a
Clone
282029
Species Reactivity
Mouse
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog. Cys25-Gly198 (Lys122Arg) Accession Number Q62226
Conjugate
Unconjugated

Applications


Application Notes
Western Blot: 2 μg/mL; Immunohistochemistry: 8-25 μg/mL; ELISA Capture: 2-8 μg/mL; ELISA Detection: 0.5-2.0 μg/mL
We recommend the following for sandwich ELISA (Capture - Detection):
DCABY-3927 - DCABY-4318
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
SHH; sonic hedgehog; Hx; Dsh; Hhg1; Hxl3
Entrez Gene ID
UniProt ID

Product Background


Pathway
Basal cell carcinoma; Dopminergic Neurogenesis; Heart Development; Hedgehog Signaling Pathway; Hedgehog signaling pathway; Pathways in cancer; PluriNetWork;

Citations


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References


Danesin, C; Agius, E; et al. Ventral neural progenitors switch toward an oligodendroglial fate in response to increased Sonic hedgehog (Shh) activity: Involvement of sulfatase 1 in modulating Shh signaling in the ventral spinal cord. JOURNAL OF NEUROSCIENCE 26:5037-5048(2006).
Khaira, SK; Nefzger, CM; et al. Midbrain and forebrain patterning delivers immunocytochemically and functionally similar populations of neuropeptide Y containing GABAergic neurons. NEUROCHEMISTRY INTERNATIONAL 59:413-420(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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