General Notes
This product (Catalog No. DPABH-28486) is a rabbit-derived polyclonal antibody raised against human SHC1, targeting the amino acid region 272–559 of the protein. This antibody exhibits cross-reactivity with Human SHC1 from mouse and rat. It has been affinity purified by Protein A. SHC1, officially known as SHC adaptor protein 1, is a protein-coding gene located on the long arm of human chromosome 1 at position 1q21.3, spanning approximately 12 kb with 15 exons. It encodes a family of adaptor proteins that play essential roles in intracellular signal transduction, particularly by linking activated receptor tyrosine kinases—such as those for epidermal growth factor (EGF) and other growth factors—to downstream signaling pathways like the RAS-MAPK cascade. The gene is ubiquitously expressed across human tissues, with highest levels in adipose tissue and the gallbladder, and produces multiple transcript variants that yield three major protein isoforms: p46Shc, p52Shc, and p66Shc, differing primarily in their N-terminal regions while sharing conserved Src homology 2 (SH2) and phosphotyrosine-binding (PTB) domains.
DPABH-28486 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-28486 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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