Anti-SCEL monoclonal antibody Made to order

Ulcerative colitis is a subtype of Inflammatory Bowel Disease. The leaves of Syzygium cumini belonging to the family Myrtaceae were selected to evaluate its efficacy in ameliorating the acetic acid-induced Ulcerative colitis. Male albino Wistar rats were randomly divided into five groups. Group 1 was given the vehicle tween 80, group II received 2 ml of 4% acetic acid solution on 8th day intrarectally, group III was given 2 ml of 4% acetic acid solution once intrarectally on 8th day and 2mg/kg of prednisolone orally for 3 days starting from the day of acetic acid treatment. Group IV and V received 7 days pretreatment with 250 mg/kg and 500 mg/kg of ethanolic leaf extract of Syzygium cumini (SCEL), respectively, and 2 ml of 4% acetic acid solution once intrarectally on 8th day. Drug treatment was continued till 10th day. The rats were sacrificed on the 11th day, and the hematological, macroscopical and biochemical parameters were assessed. Pre-treatment with the extract improved the hematological parameters, body weight, and the stool consistency score as compared to the positive control.

Papillary thyroid carcinoma (PTC) is clinically heterogeneous. Apart from an association with ionizing radiation, the etiology and molecular biology of PTC is poorly understood. We used oligobased DNA arrays to study the expression profiles of eight matched pairs of normal thyroid and PTC tissues. Additional PTC tumors and other tissues were studied by reverse transcriptase-PCR and immunohistochemistry. The PTCs showed concordant expression of many genes and distinct clustered profiles. Genes with increased expression in PTC included many encoding adhesion and extracellular matrix proteins. Expression was increased in 8/8 tumors for 24 genes and in 7/8 tumors for 22 genes. Among these genes were several previously known to be overexpressed in PTC, such as MET, LGALS3, KRT19, DPP4, MDK, TIMP1, and FN1. The numerous additional genes include CITED1, CH13L1, ODZ1, N33, SFTPB, and SCEL. Reverse transcriptase-PCR showed high expression of CITED1, CH13L1, ODZ1, and SCEL in 6/6 additional PTCs. Immunohistochemical analysis detected CITED1 and SFTPB in 49/52 and 39/52 PTCs, respectively, but not in follicular thyroid carcinoma and normal thyroid tissue. Genes underexpressed in PTC included tumor suppressors, thyroid function-related proteins, and fatty acid binding proteins. Expression was decreased in 7/8 tumors for eight genes and decreased in 6/8 tumors for 19 genes. We conclude that, despite its clinical heterogeneity, PTC is characterized by consistent and specific molecular changes. These findings reveal clues to the molecular pathways involved in PTC and may provide biomarkers for clinical use.