Anti-S1PR1 (C-terminal) polyclonal antibody

Name: Anti-S1PR1 (C-terminal) polyclonal antibody
SKU: DPAB-L21041
Rating: 5 (1 reviews)

Rabbit Anti-Human S1PR1 (C-terminal) polyclonal antibody for I-ELISA

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

a 14 amino acid peptide near the carboxy-terminus of the human S1P1.

Conjugate

Unconjugated

Target

Alternative Names

S1PR1; sphingosine-1-phosphate receptor 1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362; sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P receptor Edg-1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1; endothelial

Entrez Gene ID

1901

UniProt ID

P21453

Product Background

Antigen Description

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq, Jul 2008]

Pathway

Class A/1 (Rhodopsin-like receptors); Fc-epsilon receptor I signaling in mast cells; FoxO signaling pathway; G alpha (i) signalling events; GPCR downstream signaling; GPCR ligand binding; GPCRs; Lysosphingolipid and LPA receptors

Citations

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Documents

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References

The effect of low levels of lead (Pb) in the blood on levels of sphingosine-1-phosphate (S1P) and expression of S1P receptor 1 in the brain of the rat in the perinatal period

CHEMOSPHERE

Authors: Lukomska, A.; Baranowska-Bosiacka, I.; Budkowska, M.; Pilutin, A.; Tarnowski, M.; Dec, K.; Dolegowska, B.; Metryka, E.; Chlubek, D.; Gutowska, I.

Abstract

Sphingolipids are the main components of the lipid membrane. They also perform structural functions and participate in many signal transmission processes. One of the bioactive sphingolipids is sphingosine-1-phosphate (S1P), a ligand for five G protein-coupled receptors (S1PRs1-5), which can also act as an intracellular second messenger. S1P is responsible for the stimulation of progenitor cells in the brain, but it can also induce apoptosis of mature neurons. This study is aimed at assessing the effect of pre- and neonatal exposure to permissible Pb concentrations on SW levels and S1PR1 (EDG1) expression in the prefrontal cortex, cerebellum, and hippo campus of rats. The concentrations of S1P were determined by RP-HPLC, S1PR1 expression was determined by RT PCR and Western Blot, and receptor immunolocalization was determined by immunohistochemistry method. Our results showed that even low blood Pb concentrations, i.e. within the acceptable limit of 10 mu g/dL caused changes in the concentration of S1P in the cerebellum, prefrontal cortex, and hippocampus. Our data also showed a significant decrease in the level of S1PR1 in all studied part of brain, without significant changes in S1PR1 gene expression. Pre- and neonatal exposure to Pb also resulted in a decrease in the expression of S1PR1 in glial cells in all regions of the Cornu Ammonis (CM-CA4) and Dentate Gyrus in the hippocampus, as well as in all layers of the cerebellum and prefrontal cortex, compared to the unexposed control group. (C) 2016 Elsevier Ltd. All rights reserved.

Effect of sphingosine-1-phosphate on cryopreserved sheep testicular explants cultured in vitro

THERIOGENOLOGY

Authors: Singh, Ram Pratap; Escobar, Enrique; Wildt, David; Patel, Seema; Costa, Guilherme M. J.; Pukazhenthi, Budhan

Abstract

Complete spermatogenesis has been achieved in vitro in mouse testicular explants with resulting sperm used to produce pups after Intra Cytoplasm Sperm Injection and Embryo Transfer. In the present study, we evaluated the influence of sphingosine-l-phosphate (SIP) on spermatogenesis of frozen-thawed lamb testis explants in vitro. Thawed testicular pieces were cultured for 12 d on agarose blocks in serum-free growth medium containing 0, 2, 5 or 10 mu M SIP. At the end of D6 and D12, some pieces were fixed and processed for histology. Other pieces were processed for RNA isolation and quantitation of proliferation (PCNA, Ki67) and differentiation (PLZF) markers and genes involved in SIP signaling (S1PR1, SGPL1, SGPP1, AKT1 and NFKBIA) by qPCR. Histology revealed an increase (P < 0.05) in seminiferous cord (SC) diameter under all culture conditions, except 5 and 10 mu M SIP by D6. In the presence of 5 mu M SIP, percentage of gonocytes decreased (P < 0.05) by D6 (control, 24.9% vs. SIP, 10.3%) with a concomitant increase (P < 0.05) in spermatogonia formation (control, 74.4% vs. SIP, 88.1%). SIP induced PCNA or Ki67 expression by D6, whereas PLZF was up-regulated (P < 0.05) by D6 in 2 mu M SIP and D12 in 5 & 10 mu M S1P. Expression of SGPL1 and SGPP1 increased 4-12-fold in tissues cultured in 10 mu M SIP by D12 compared to D12 control. AKT1 and NFKBIA mRNA expression was low (P < 0.05) in 5 and or 10 mu M SIP treatments on D6. These results demonstrate that SIP promotes germ cell proliferation during first week of culture and may exert an anti-apoptotic influence on the seminiferous cord in sheep testicular explants in vitro. (C) 2019 Elsevier Inc. All rights reserved.