This product (Catalog No. DPABH-03525) is a rabbit-derived polyclonal antibody raised against human RFFL. This antibody exhibits cross-reactivity with RFFL from mouse and rat. It has been affinity purified by Protein A. Enables enzyme binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in negative regulation of signal transduction; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein K48-linked ubiquitination. Located in endosome membrane and plasma membrane. DPABH-03525 is suitable for use in Western blot (WB) (1:500–1:2,000) application. DPABH-03525 is supplied as a liquid formulation in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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