Rabbit Anti-Human RPL22 (center) Polyclonal antibody

Name: Rabbit Anti-Human RPL22 (center) Polyclonal antibody
SKU: DPABH-17855
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Rabbit Anti-Human RPL22 (center) Polyclonal antibody for WB, IHC

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COA

SDS

Datasheet

Key features and details

Target: RPL22
Host: Rabbit
Application: WB, IHC
Species Reactivity: Human, Mouse, Rat

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL22.

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-1:1000
IHC: 1:100-1:200

General Notes

This product (Catalog No. DPABH-17855) is a rabbit-derived polyclonal antibody raised against human RPL22. This antibody exhibits cross-reactivity with RPL22 from mouse and rat. It has been affinity purified by Protein A. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2.
DPABH-17855 is suitable for use in Western blot (WB) (1:500–1:1,000), IHC (1:100–1:200) applications.
DPABH-17855 is supplied as a liquid formulation in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

60S ribosomal protein L22; EBER-associated protein; EAP; Epstein-Barr virus small RNA-associated protein; Heparin-binding protein HBp15

Entrez Gene ID

6146

UniProt ID

P35268

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

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References

Interaction of casein kinase II with ribosomal protein L22 of Drosophila melanogaster

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Authors: Zhao, W; Bidwai, AP; Glover, CVC

Abstract

The ubiquitous eukaryotic protein kinase CKII (casein kinase II) has been found to interact with a number of cellular proteins, either through the catalytic subunit or the regulatory subunit. Using the yeast two-hybrid screening method, we found that the catalytic subunit of Drosophila melanogaster CKII (DmCKII) interacts with Drosophila ribosomal protein L22 (rpL22). This interaction was also observed in vitro with a glutathione-S-transferase (GST)-rpL22 fusion protein. The predicted full-length Drosophila rpL22 protein has an N-terminal extension rich in alanine, lysine, and proline that appears to be unique to Drosophila. Deletion mapping revealed that the conserved core of rpL22 is responsible for the interaction with CKII. Moreover, purified DmCKII can phosphorylate a GST-L22 fusion protein at the C-terminal end, suggesting that this protein may be a substrate of CKII in Drosophila. (C) 2002 Elsevier Science (USA). All rights reserved.

Complete chloroplast genome of Caragana intermedia (Fabaceae), an endangered shrub endemic to china

CONSERVATION GENETICS RESOURCES

Authors: Zhang, Zi-long; Ma, Li-ying; Yao, Hong-bin; Yang, Xin; Luo, Ji-huai; Gong, Xue; Wei, Shuo-yue; Li, Quan-fang; Wang, Wei; Sun, Hong-bin

Abstract

Caragana intermedia is an important shrub plant with high ecological and economic value. Here, we reported and analyzed the complete chloroplast (cp) genome of C. intermedia. This cp genome is relatively short (129,331 bp) because it lacks an Inverted Repeat (IR) region. It comprises 111 genes, including four unique rRNAs, 31 tRNAs, and 76 protein-coding genes. Similar to other closely related plastomes, rpl22 and rps16 are absent, and the GC content is relatively low (31.6 %). The phylogenetic analysis suggested that C. intermedia is closely related to the genera of Astragalus.