Anti-RPL10A monoclonal antibody, clone FQS23455

The relative expression of eleven egg performance-associated genes including the tau-crystallin/alpha enolase (ENO1), ribosomal protein S27a (RPS27A), calmodulin 1 (CALM1), solute carrier family 25 A6 (SLC25A6), arylsulfatase A (ARSA), syndecan 2 (SDC2), ribosomal protein L10 alpha (RPL10A), ornithine decarboxylase antizyme 1 (OAZ1), cytochrome c somatic variant 2 (CYCS), adenosine triphosphate synthase, H+ transporting, alpha subunit, isoform 1 (ATP5A1) and the ring finger protein 130 (RNF130) in the ovaries of pre-laying and laying Zi geese were analyzed using quantitative real-time PCR (qRT-PCR). Comparison between pre-laying and egg-laying stages, the relative expression of ENO1, RPS27A, SLC25A6, ARSA, SDC2, RPL10A, OAZ1, CYCS and RNF130 were significantly upregulated (P<0.05), whereas the CALM1 and ATP5A1 were highly significantly up-regulated (P < 0.01). This study established the primary foundation to understand the possible roles of these genes in the ovaries.

Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.