Anti-RPA3 monoclonal antibody, clone 22.2

Antibody Isotype

IgG1

Clone

22.2

Species Reactivity

Mouse; Human

Immunogen

N-terminally 6x-His tagged fusion protein encoding full-length human RPA-14 expressed in E. coli.

Conjugate

Application Notes

WB: 0.1 - 2 μg/ml; IP: 0.1 - 2 μg/ml; Flow Cyt: 1μg/10⁶ cells;

Alternative Names

RPA3; replication protein A3, 14kDa; replication protein A3 (14kD); replication protein A 14 kDa subunit; REPA3; RP-A p14; RF-A protein 3; replication factor A protein 3;

Entrez Gene ID

UniProt ID

Antigen Description

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Pathway

Activation of ATR in response to replication stress, organism-specific biosystem; Activation of the pre-replicative complex, organism-specific biosystem; Assembly of the RAD51-ssDNA nucleoprotein complex, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem;