Anti-RPA1 monoclonal antibody, clone 9D4-E23-I21

Antibody Isotype

IgG2a

Clone

9D4-E23-I21

Species Reactivity

Human

Immunogen

Recombinant fragment corresponding to Escherichia coli RPA70 (C terminal).

Conjugate

Alternative Names

RPA1; replication protein A1; replication protein A 70 kDa DNA-binding subunit; RP-A p70; RF-A protein 1; replication factor A protein 1; Rpa; RF-A; RP-A; 70kDa; AA589576; AW557552; 5031405K23Rik;

Entrez Gene ID

UniProt ID

Antigen Description

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Pathway

Activation of ATR in response to replication stress, organism-specific biosystem; Activation of the pre-replicative complex, organism-specific biosystem; Assembly of the RAD51-ssDNA nucleoprotein complex, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem;