Rabbit Anti-RHO Polyclonal Antibody (DPABH-20528)

Rabbit Anti-Human RHO Polyclonal Antibody for WB Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human RHO (NP_000530.1).
Conjugate
unconjugated

Applications


Application Notes
WB:    1:500 - 1:2000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Western blot analysis of extracts of various cell lines, using RHO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Target


Alternative Names
RHO; rhodopsin; RP4; OPN2; CSNBAD1; opsin-2
Entrez Gene ID
UniProt ID

Citations


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References


Nakashima, M; Adachi, S; et al. Inhibition of Rho-associated coiled-coil containing protein kinase enhances the activation of epidermal growth factor receptor in pancreatic cancer cells. MOLECULAR CANCER 10:-(2011).
Ma, MZ; Zhuang, C; et al. CTHRC1 Acts as a Prognostic Factor and Promotes Invasiveness of Gastrointestinal Stromal Tumors by Activating Wnt/PCP-Rho Signaling. NEOPLASIA 16:-(2014).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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