Rabbit Anti-RHO Polyclonal Antibody (DPABH-20528)
Rabbit Anti-Human RHO Polyclonal Antibody for WB
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Human, Mouse, Rat
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human RHO (NP_000530.1).
WB: 1:500 - 1:2000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of extracts of various cell lines, using RHO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
RHO; rhodopsin; RP4; OPN2; CSNBAD1; opsin-2
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Junemann, A; Winterhoff, M; et al. ForC lacks canonical formin activity but bundles actin filaments and is required for multicellular development of Dictyostelium cells. EUROPEAN JOURNAL OF CELL BIOLOGY 92:201-212(2013).
Morita, K; Taketomi, A; et al. Clinical significance and potential of hepatic microRNA-122 expression in hepatitis C. LIVER INTERNATIONAL 31:474-484(2011).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.