Rabbit Anti-Human RGS12 (N-terminal) Polyclonal antibody

Name: Rabbit Anti-Human RGS12 (N-terminal) Polyclonal antibody
SKU: DPABH-01333
Rating: 5 (1 reviews)

Rabbit Anti-Human RGS12 (N-terminal) Polyclonal antibody for WB

COA

SDS

Datasheet

Key features and details

Target: RGS12
Host: Rabbit
Application: WB
Species Reactivity: Human, Mouse

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

Synthesized peptide derived from N-terminal of human RGS12.

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-1:3000

General Notes

This product (Catalog No. DPABH-01333) is a rabbit-derived IgG polyclonal antibody raised against human RGS12. This antibody exhibits cross-reactivity with RGS12 from mouse. It has been affinity purified by protein A. This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined.
DPABH-01333 is suitable for use in Western blot (WB) (1:500–1:3,000) application.
DPABH-01333 is supplied as a liquid formulation in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

regulator of G-protein signaling 12

Entrez Gene ID

6002

UniProt ID

O14924

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Discovery of susceptibility loci associated with tuberculosis in Han Chinese

HUMAN MOLECULAR GENETICS

Authors: Qi, Hui; Zhang, Yong-Biao; Sun, Lin; Chen, Cheng; Xu, Biao; Xu, Fang; Liu, Jia-Wen; Liu, Jin-Cheng; Chen, Chen; Jiao, Wei-Wei; Shen, Chen; Xiao, Jing; Li, Jie-Qiong; Guo, Ya-Jie; Wang, Yong-Hong; Li, Qin-Jing; Yin, Qing-Qin; Li, Ying-Jia; Wang, Ting; Wang, Xing-Yun; Gu, Ming-Liang; Yu, Jun; Shen, A-Dong

Abstract

Genome-wide association studies (GWASs) have revealed the worldwide heterogeneity of genetic factors in tuberculosis (TB) susceptibility. Despite having the third highest global TB burden, no TB-related GWAS has been performed in China. Here, we performed the first three-stage GWAS on TB in the Han Chinese population. In the stage 1 (discovery stage), after quality control, 691 388 SNPs present in 972 TB patients and 1537 controls were retained. After replication on an additional 3460 TB patients and 4862 controls (stages 2 and 3), we identified three significant loci associated with TB, the most significant of which was rs4240897 (logistic regression P = 1.41 x 10(-11), odds ratio = 0.79). The aforementioned three SNPs were harbored by MFN2, RGS12 and human leukocyte antigen class II beta chain paralogue encoding genes, all of which are candidate immune genes associated with TB. Our findings provide new insight into the genetic background of TB in the Han Chinese population.

RGS12 Is a Novel Tumor-Suppressor Gene in African American Prostate Cancer That Represses AKT and MNX1 Expression

CANCER RESEARCH

Authors: Wang, Yongquan; Wang, Jianghua; Zhang, Li; Karatas, Omer Faruk; Shao, Longjiang; Zhang, Yiqun; Castro, Patricia; Creighton, Chad J.; Ittmann, Michael

Abstract

African American (AA) men exhibit a relatively high incidence and mortality due to prostate cancer even after adjustment for socioeconomic factors, but the biological basis for this disparity is unclear. Here, we identify a novel region on chromosome 4p16.3 that is lost selectively in AA prostate cancer. The negative regulator of G-protein signaling RGS12 was defined as the target of 4p16.3 deletions, although it has not been implicated previously as a tumor-suppressor gene. RGS12 transcript levels were relatively reduced in AA prostate cancer, and prostate cancer cell lines showed decreased RGS12 expression relative to benign prostate epithelial cells. Notably, RGS12 exhibited potent tumor-suppressor activity in prostate cancer and prostate epithelial cell lines in vitro and in vivo. We found that RGS12 expression correlated negatively with the oncogene MNX1 and regulated its expression in vitro and in vivo. Further, MNX1 was regulated by AKT activity, and RGS12 expression decreased total and activated AKT levels. Our findings identify RGS12 as a candidate tumor-suppressor gene in AA prostate cancer, which acts by decreasing expression of AKT and MNX1, establishing a novel oncogenic axis in this disparate disease setting. (C) 2017 AACR.