Applications: WB Reactive species: Mouse
"Abstract: Cell-type-specific splicing depends on RNA-binding splicing factors. Several important splicing factors were identified in cardiomyocytes, including members of the RNA-binding proteins with multiple splicing (RBPMS) family, but their role during heart development has not been fully characterized. Here, we demonstrate that the function of RBPMS overlaps with the closely related paralog RBPMS2. Rbpms-deficient cardiomyocytes exhibit a higher degree of binucleation at birth, but this does not affect heart function in mice substantially until late adulthood. In contrast, Rbpms/Rbpms2 (Rbpms/2) compound mutants show pronounced disruption of the splicing network in embryonic cardiomyocytes, which leads to the formation of defective nuclei and disruption of sarcomere structures, eventually resulting in embryonic lethality. We demonstrate that mitotic defects in embryonic Rbpms/2-deficient cardiomyoctes are caused by the disbalance of nuclear and cytoplasmic calcium (Ca2+)/CaM-dependent protein kinase II gamma (Camk2g) isoforms. Overexpression of the Rbpmsa isoform partially rescues these defects, preventing embryonic lethality of Rbpms/2-deficient mice, and is sufficient for cardiomyocyte-specific splicing in other cell types." Article snippet: The following primary antibodies concentrations were used RBPMS 1:1000 (***), RBPMS2 1:1000 (Creative Diagnostics; DCABH-7076) and GAPDH 1:3000 (***). Rabbit monoclonal anti-RBPMS2 antibody, Creative Diagnostics, Cat# DCABH-7076; RRID:AB_2480444
Figure 1. Western blot analysis of RBPMS and GFP protein levels in different tissues of control and Rbpmsa-Gfp mice.
My Review for Anti-RBPMS2 monoclonal antibody, clone FQS24232-80
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
COA
Publication 
Loading ......
