Rabbit Anti-Human RBP1 (a.a. 64-135) Polyclonal antibody

Name: Rabbit Anti-Human RBP1 (a.a. 64-135) Polyclonal antibody
SKU: DPABH-04725
Rating: 5 (1 reviews)

Rabbit Anti-Human RBP1 (a.a. 64-135) Polyclonal antibody for ELISA, IHC, WB

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COA

SDS

Datasheet

Key features and details

Target: Human RBP1
Host: Rabbit
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse, Rat

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

E. coli - derived recombinant Human RBP1 (Asp64-Gln135).

Conjugate

Unconjugated

Applications

Application Notes

ELISA: 1:4000-1:8000
IHC: 1:50-1:100
WB: 1:1000-1:4000

General Notes

This product (Catalog No. DPABH-04725) is a rabbit-derived polyclonal antibody raised against human RBP1, targeting the amino acid region 64–135 of the protein. This antibody exhibits cross-reactivity with Human RBP1 from mouse and rat. It has been affinity purified by Protein A. Retinol-binding protein 1 (RBP1), also known as cellular retinol-binding protein 1 (CRBP1), is a protein encoded by the RBP1 gene in humans, located on chromosome 3q23. This protein serves as an intracellular carrier that facilitates the transport, storage, and metabolism of retinol (vitamin A alcohol) within cells, playing a crucial role in maintaining retinoid homeostasis essential for processes such as vision, growth, reproduction, and epithelial cell differentiation. Unlike retinol-binding protein 4 (RBP4), which handles plasma transport of retinol from the liver to peripheral tissues, RBP1 operates intracellularly and interacts with membrane receptors like STRA6 to support retinol uptake.
DPABH-04725 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-04725 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

RBP1; Cellular retinol-binding protein I; Retinol-binding protein 1; CRBP1; CRBP; Cellular retinol-binding protein; CRBP-I

UniProt ID

P09455

Citations

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Documents

pdf COA

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pdf Datasheet

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References

ALTERNATIVE SPLICING OF THE RBP1 GENE CLUSTERS IN AN INTERNAL EXON THAT ENCODES POTENTIAL PHOSPHORYLATION SITES

ONCOGENE

Authors: OTTERSON, GA; KRATZKE, RA; LIN, AY; JOHNSTON, PG; KAYE, FJ

Abstract

We have isolated cDNA and genomic clones for the human retinoblastoma binding protein 1 (RBP1) gene, and have identified alternative splicing of RBP1 clustered within a 207-nucleotide internal exon. Three of the predicted RPB1 peptides share amino-terminal and carboxy-terminal domains, while a fourth species encodes a distinct carboxy-terminal domain. Functional analysis of these peptides demonstrated that they are capable of precipitating retinoblastoma (RB) protein in vitro from K562 cell lysates, but cannot bind to mutant RB protein. However, each of the RBP1 peptides differed within an internal exon that contains potential casein kinase II and p34cdc2 phosphorylation sites. Immunoblot analysis using polyclonal alpha-RBP1 antiserum revealed that the RBP1 protein is expressed in a wide range of cell lines of differing histologic type and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis predominantly as a 200-kDa protein. Immunohistochemical analysis using the alpha-RBP1 antiserum demonstrated a distinct nuclear staining pattern that was eliminated when the antiserum was preabsorbed with RBP1 peptide. The RBP1 gene encodes a widely expressed 200-kDa nuclear protein and undergoes alternative splicing that predicts a family of RB-binding peptides.

Annexin-A1 restricts Th17 cells and attenuates the severity of autoimmune disease

JOURNAL OF AUTOIMMUNITY

Authors: Yazid, Samia; Gardner, Peter J.; Carvalho, Livia; Chu, Colin J.; Flower, Roderick J.; Solito, Egle; Lee, Richard W. J.; Ali, Robin R.; Dick, Andrew D.

Abstract

Annexin-A1 (Anx-A1) is an endogenous anti-inflammatory molecule and while described as a repressor of innate immune responses, the role of Anx-A1 in adaptive immunity, and in particular in T helper (Th) cell responses, remains controversial. We have used a T-cell mediated mouse model of retinal autoimmune disease to unravel the role of Anx-A1 in the development of autoreactive Th cell responses and pathology. RBP1-20-immunized C57BL/6 Anx-A1(-/-) mice exhibit significantly enhanced retinal inflammation and pathology as a result of an uncontrolled proliferation and activation of Th17 cells. This is associated with a limited capacity to induce SOCS3, resulting in un-restricted phosphorylation of STAT3. RBP1-20-specific CD4(+) cells from immunized Anx-A1-/- animals generated high levels of Th17 cells-associated cytokines. Following disease induction, daily systemic administration of human recombinant Anx-A1 (hrAnx-A1), during the afferent phase of disease, restrained autoreactive CD4+ cell proliferation, reduced expression of pro-inflammatory cytokines IL-17, IFN-gamma and IL-6 and attenuated autoimmune retinal inflammatory disease. Furthermore, in man, Anx-A1 serum levels when measured in active uveitis patient sera were low and associated with the detection of IgM and IgG anti-Anx-A1 antibodies when compared to healthy individuals. This data supports Anx-A1 as an early and critical regulator of Th17 cell driven autoimmune diseases such as uveitis. (C) 2014 Elsevier Ltd. All rights reserved.