Anti-RBM8A monoclonal antibody, clone FQS24056(C)

Antibody Isotype

IgG

Clone

FQS24056(C)

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Y14 aa 1-100 (Cysteine residue). The exact sequence is proprietary.Database link: Q9Y5S9

Conjugate

Application Notes

WB: 1/1000 - 1/10000; IHC-P: 1/250 - 1/500; ICC/IF: 1/250 - 1/500; Flow Cyt: 1/10 - 1/100; IP: 1/10 - 1/100;

Alternative Names

RBM8A; RNA binding motif protein 8A; RBM8; RNA-binding protein 8A; BOV 1A; BOV 1B; BOV 1C; RBM8B; Y14; ZNRP; BOV-1; binder of OVCA1-1; ribonucleoprotein RBM8; RNA-binding protein Y14; ribonucleoprotein RBM8A; RNA binding motif protein 8B; RNA-binding moti

Entrez Gene ID

UniProt ID

Antigen Description

Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.

Pathway

Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Exon junction complex (EJC), organism-specific biosystem; Gene Expression, organism-specific biosystem; Nonsense Mediated Decay Enhanced by the Exon Junction Complex, organism-specific biosystem; Nonsense-Mediated Decay, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem;