Recombinant funsion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2).
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500 - 1:2000
Images
Western blot analysis of extracts of various cell lines, using PUS10 antibody at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More
Citations
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Chatterjee, K; Blaby, IK; et al. The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA. RNA-A PUBLICATION OF THE RNA SOCIETY 18:421-433(2012).
McCleverty, CJ; Hornsby, M; et al. Crystal structure of human Pus10, a novel pseudouridine synthase. JOURNAL OF MOLECULAR BIOLOGY 373:1243-1254(2007).
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Rabbit Anti-PUS10 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PUS10 antibody at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
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