Rabbit Anti-Human PTRF Polyclonal antibody

Structure of Mo species deposited on a TiO2(110) surface has been studied using in situ polarization-dependent total reflection fluorescence EXAFS (PTRF-EXAFS). We found anisotropic ordering of the Mo species reflecting the anisotropic structure of TiO2 surface when Mo(CO)(6) was deposited under reductive conditions. Mo chains were produced with its Mo-Mo bonding parallel to the [001]direction of the TiO2 substrate. The results provide a possible way to regulate the structure of Mo species on TiO2(110) by the preparation conditions. (C) 2001 Elsevier Science B.V. All rights reserved.

IntroductionGlioblastoma (GBM) is the most common primary brain cancer. The average survival time for the majority of patients is approximately 15months after diagnosis. A major feature of GBM that contributes to its poor prognosis is its high invasiveness. Caveolae are plasma membrane subdomains that participate in numerous biological functions. Caveolin-1 and Caveolae Associated Protein 1 (CAVIN1), formerly termed Polymerase I and Transcript Release Factor, are both necessary for caveola formation. We hypothesized that high expression of caveola-forming proteins in GBM promotes invasiveness via modulation of the production of matrix-degrading enzymes.MethodsThe mRNA expression of caveola-forming proteins and matrix proteases in GBM samples, and survival after stratifying patients according to caveolin-1 or CAVIN1 expression, were analyzed from TCGA and REMBRANDT databases. The proteolytic profile of cell lines expressing or devoid of caveola-forming proteins was investigated using zymography and real-time qPCR. Invasion through basement membrane-like protein was investigated in vitro.ResultsExpression of both caveolin-1 and CAVIN1 was increased in GBM compared to normal samples and correlated with expression of urokinase plasminogen activator (uPA) and gelatinases. High expression of caveola-forming proteins was associated with shorter survival time. GBM cell lines capable of forming caveolae expressed more uPA and matrix metalloproteinase-2 (MMP-2) and/or -9 (MMP-9) and were more invasive than GBM cells devoid of caveola-forming proteins. Experimental manipulation of caveolin-1 or CAVIN1 expression in GBM cells recapitulated some, but not all of these features. Caveolae modulate GBM cell invasion in part via matrix protease expression.