General Notes
This product (Catalog No. DPABH-05712) is a rabbit-derived IgG polyclonal antibody raised against human PSMD12, targeting the amino acid region 101–200 of the protein. This antibody exhibits cross-reactivity with PSMD12 from mouse and rat. It has been affinity purified by Protein A. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
DPABH-05712 is suitable for use in Western blot (WB) (1:500–1:2,000), IHC-P (1:100–1:500), IHC-F (1:100–1:500), IF (immunofluorescence) (1:100–1:500), ICC/IF (1:100–1:500), ELISA (1:5,000–1:10,000) applications.
DPABH-05712 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
Alternative Names
26S Proteasome regulatory subunit p55; 26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit p55; 26S proteasome regulatory subunit RPN5; MGC75406; p55; proteasome (prosome, macropain) 26S subunit, non ATPase12; Proteasome 26S non ATPase subunit 12 isoform 2; PSD12_HUMAN; PSMD12; Rpn5
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