Application: WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Species Reactivity: Human, Mouse, Rat
Summary
Specifications
Antibody Isotype
IgG
Species Reactivity
Human; Mouse; Rat
Immunogen
Recombinant human PRKAR2B protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 1-122 aa of BC075800
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500-1:2000 IP: 0.5-4.0 μg for 1.0-3.0 mg of total protein lysate IHC: 1:50-1:500 IF/ICC: 1:200-1:800 FC: 0.40 μg per 10^6 cells in a 100 μl suspension
General Notes
This product (Catalog No. DPABH-04592) is a rabbit-derived polyclonal antibody raised against human PRKAR2B, targeting the amino acid region 1–122 of the protein. This antibody exhibits cross-reactivity with Human PRKAR2B from mouse and rat. It has been affinity purified by Protein A. Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. DPABH-04592 is suitable for use in Western blot (WB) (1:500–1:2,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:50–1:500), IF/ICC (1:200–1:800), flow cytometry (FC) (0.40 μg per 10^6 cells in a 100 μl suspension) applications. DPABH-04592 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
Target
Alternative Names
cAMP-dependent protein kinase type II-beta regulatory subunit; PRKAR2; RII BETA
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