Anti-PRDX4 monoclonal antibody, clone 21B2

Antibody Isotype

IgG1

Clone

21B2

Species Reactivity

Human

Immunogen

Sulfonylated synthetic peptide corresponding to active site of mammalian Prx I to IV conjugated to KLH.

Conjugate

Application Notes

WB: 1/100 - 1/500; Flow Cyt: 1μg/10⁶ cells; ICC/IF: 10 μg/ml.

Alternative Names

PRDX4; peroxiredoxin 4; peroxiredoxin-4; AOE37 2; prx-IV; peroxiredoxin IV; antioxidant enzyme AOE372; thioredoxin peroxidase AO372; thioredoxin peroxidase (antioxidant enzyme); thioredoxin-dependent peroxide reductase A0372; PRX-4; AOE37-2;

Entrez Gene ID

UniProt ID

Antigen Description

Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.

Pathway

Selenium Pathway, organism-specific biosystem;