Anti-POLR3C monoclonal antibody, clone 3I2

Antibody Isotype

IgG1

Clone

3I2

Species Reactivity

Human

Immunogen

Recombinant full length protein, corresponding to amino acids 1-534 of Human RPC62 produced in HEK293T cells (NP_006459).

Conjugate

Application Notes

WB: 1/2000; IHC-P: 1/150; ICC/IF: 1/100;

Alternative Names

POLR3C; polymerase (RNA) III (DNA directed) polypeptide C (62kD); DNA-directed RNA polymerase III subunit RPC3; RPC3; RPC62; RNA polymerase III subunit C3; RNA polymerase III 62 kDa subunit; DNA-directed III 62 kDa polypeptide; DNA-directed RNA polymerase

Entrez Gene ID

UniProt ID

Antigen Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Pathway

Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Gene Expression, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem;