Anti-POLR2E monoclonal antibody (DCABH-615)

Mouse anti-Human POLR2E monoclonal antibody for WB, ICC/IF Datasheet

Bring this labeled antibody directly to your bench!

Online Inquiry Add to basket

Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Clone
4D6
Species Reactivity
Human
Immunogen
Recombinant full length Human POLR2E produced in HEK293T cells (NP_002686).
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/500 - 1/2000; ICC/IF: 1/100;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
POLR2E; polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; polymerase (RNA) II (DNA directed) polypeptide E (25kD); DNA-directed RNA polymerases I, II, and III subunit RPABC1; DNA directed RNA polymerase II 23 kda polypeptide; hRPB25
Entrez Gene ID
UniProt ID

Product Background


Gene summary
POLR2E (Polymerase (RNA) II Subunit E) is a Protein Coding gene. Among its related pathways are Gene Expression and Signaling by GPCR. GO annotations related to this gene include DNA-directed RNA polymerase activity and RNA polymerase I activity. This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.
Antigen Description
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Seems to be the major component in this process (By similarity). DNA-directed RNA polymerases I, II, and III subunit RPABC1 is a protein that in humans is encoded by the POLR2E gene. 0The function about POLR2E antigen include DNA binding; DNA-directed RNA polymerase activity; protein binding; contributes_to protein kinase activity.
Pathway
Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem.

Citations


Have you cited DCABH-615 in a publication? Let us know and earn a reward for your research.

References


Mita, P; Savas, JN; et al. Analysis of URI Nuclear Interaction with RPB5 and Components of the R2TP/Prefoldin-Like Complex. PLOS ONE 8:-(2013).
Wada, T; Yamashita, Y; et al. Screening for genetic abnormalities involved in ovarian carcinogenesis using retroviral expression libraries. INTERNATIONAL JOURNAL OF ONCOLOGY 35:973-976(2009).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

Customer Reviews


Write a review, share your experiences with others and get rewarded !

Online Inquiry

Name
Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * Please input "diagnostics" as verification code.

Online Inquiry

Order Info: Anti-POLR2E monoclonal antibody

Online Inquiry
  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!
  15% off your first purchase

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket