Anti-PIKFYVE Monoclonal antibody, clone 4E3

Antibody Isotype

IgG1

Clone

4E3

Species Reactivity

Human

Immunogen

PIP5K3 (NP_689884, 342 a.a.~452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Application Notes

WB: 1:500-1000

Alternative Names

PIKFYVE; phosphoinositide kinase, FYVE finger containing; phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase, type III , PIP5K3; 1-phosphatidylinositol 3-phosphate 5-kinase; KIAA0981; MGC40423; p235; PIKfyve; PIP5K; PIPkin-III; type III PIP ki

Entrez Gene ID

UniProt ID

Antigen Description

Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Pathway

3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Phagosome, organism-specific biosystem; Phagosome, conserved biosystem; Phosphatidylinositol signaling system, organism-specific biosystem;