Rabbit Anti-Human PIGT (a.a. 230-578) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human; Mouse

Immunogen

Recombinant human PIGT protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 230-578 aa of BC015022

Conjugate

Application Notes

WB: 1:500-1:1000
IP: 0.5-4.0 μg for 1.0-3.0 mg of total protein lysate
IHC: 1:100-1:500
IF/ICC: 1:50-1:500

General Notes

This product (Catalog No. DPABH-18076) is a rabbit-derived polyclonal antibody raised against human PIGT, targeting the amino acid region 230–578 of the protein. This antibody exhibits cross-reactivity with Human PIGT from mouse. It has been affinity purified by Protein A. PIGT is a subunit of the glycosylphosphatidylinositol transamidase complex that catalyzes the attachment of proteins to GPI-anchors. GPI-anchors are glycolipids found on membrane of diverse cells that help proteins anchoring to the cell surface. Mutations of PIGT have been linked to developmental disorders including multiple congenital anomalies-hypotonia-seizures syndrome-3. Multiple isoforms of PIGT exist due to the alternative splicing, with the predicted MWs ranging from 42 kDa to 66 kDa.
DPABH-18076 is suitable for use in Western blot (WB) (1:500–1:1,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:100–1:500), IF/ICC (1:50–1:500) applications.
DPABH-18076 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

CGI-06; GPI transamidase component PIG-T; NDAP; Phosphatidylinositol-glycan biosynthesis class T protein; PSEC0163

Entrez Gene ID

UniProt ID