Rabbit Anti-Human PHGDH (a.a. 1-533) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

E. coli - derived recombinant Human PHGDH (Met1-Phe533).

Conjugate

Application Notes

ELISA: 1:4000-1:8000
IHC: 1:50-1:100
WB: 1:1000-1:4000

General Notes

This product (Catalog No. DPABH-25217) is a rabbit-derived polyclonal antibody raised against human PHGDH, targeting the amino acid region 1–533 of the protein. It has been affinity purified by Protein A. Phosphoglycerate dehydrogenase (PHGDH), also known as 3-phosphoglycerate dehydrogenase, is an NAD/NADH-dependent enzyme that catalyzes the first and rate-limiting step in the phosphorylated pathway of L-serine biosynthesis, converting 3-phosphoglycerate to 3-phosphohydroxypyruvate. Encoded by the PHGDH gene located on human chromosome 1p12, the enzyme forms homotetramers and is highly expressed in tissues such as the brain, liver, kidney, and proliferating cells, including those in tumors. L-serine produced via this pathway is essential for one-carbon metabolism, protein and nucleotide synthesis, myelin formation, and cellular redox balance, playing critical roles in central nervous system development and cellular proliferation.
DPABH-25217 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-25217 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

2-oxoglutarate reductase; Malate dehydrogenase; 3-PGDH; PGDH3; PHGDH; D-3-phosphoglycerate dehydrogenase

UniProt ID