General Notes
This product (Catalog No. DPABH-17087) is a rabbit-derived polyclonal antibody raised against human PHF1, targeting the amino acid region 1–351 of the protein. This antibody exhibits cross-reactivity with Human PHF1 from mouse and rat. It has been affinity purified by Protein A. Plant homeodomain finger protein 1 (PHF1) has a specific role in histone modification and in non-homologous end joining. It is a human homolog of the Drosophila protein Polycomb-like protein. PHF1 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. PHF1 regulates also p53/TP53 stability. PHF1 contains two zinc finger-like plant homeodomain (PHD) sequences, a Cys4-His-Cys3 motif that is able to recognize and modify histone tails, making it important in epigenetic regulation. It also contains a Tudor domain, a sequence of approximately 60 amino acids that has also been shown to bind to methylated histone tails. The sequence of PHF1 is similar to the Polycomb family of genes in Drosophila, chromatin remodeling genes that act in embryogenesis. PHF1 has a key role in histone modification. It acts by binding to the histone H3K36me3, a lysine methylation of histone H3 that is a mark for transcriptional activation. PHF1 then recruits Polycomb repressive complex 2 (PRC2) to mark genes for repression. These epigenetic chromatin modifiers are important in cancer development, gene silencing, and stem cell maintenance. PHF1 is also found to be recruited to double-strand breaks in DNA and plays an important part in the repair in non-homologous end joining, the main DNA repair process in which ends of DNA are ligated without a homologous template. Interacting with proteins involved in DNA damage response, PH1 appears to have a role beyond the polycomb function.
DPABH-17087 is suitable for use in Western blot (WB) (1:1,000–1:6,000), IHC (1:250–1:1,000) applications.
DPABH-17087 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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