Anti-PHAX (aa 304-334) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Synthetic peptide within Human PHAX aa 304-334 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.Database link: Q9H814

Conjugate

Application Notes

IHC-P: 1/10 - 1/50; WB: 1/100 - 1/500.

Alternative Names

PHAX; phosphorylated adaptor for RNA export; RNA U, small nuclear RNA export adaptor (phosphorylation regulated); RNUXA; phosphorylated adapter RNA export protein; FLJ13193; RNA U small nuclear RNA export adapter protein; RNA U, small nuclear RNA export a

Entrez Gene ID

UniProt ID

Antigen Description

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

Pathway

Gene Expression; Metabolism of non-coding RNA; RNA transport; snRNP Assembly;