General Notes
This product (Catalog No. DPABH-01675) is a rabbit-derived polyclonal antibody raised against human PDF, targeting the amino acid region 7–243 of the protein. It has been affinity purified by Protein A. Peptide deformylase (PDF) is a metalloprotease enzyme, typically around 20 kDa in size, that catalyzes the hydrolytic removal of the N-formyl group from the N-terminal formylmethionine residue of nascent polypeptides during protein biosynthesis. This deformylation step is essential for the co-translational maturation of proteins, unmasking the amino group of the initiator methionine to allow subsequent cleavage by methionine aminopeptidase (MAP), and it affects the processing, stability, and function of a majority of cellular proteins. PDF operates via a conserved active site featuring a HEXXH motif that binds a catalytic metal ion, such as zinc or nickel, hydrolyzing the formyl-methionine amide bond with specificity for small second residues like alanine or serine. The enzyme belongs to the superfamily of zinc metalloproteases and is characterized by two signature sequences essential for its activity.
DPABH-01675 is suitable for use in IHC (1:100–1:400), IF/ICC (1:200–1:800) applications.
DPABH-01675 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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