Anti-PCF11 monoclonal antibody (DCABH-691)

Mouse anti-Human PCF11 monoclonal antibody for WB, ELISA, sELISA Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG2a
Species Reactivity
Human
Immunogen
Recombinant fragment, corresponding to C terminal amino acids 1465-1555 of Human PCF11 with proprietary tag (NP_056969).
Conjugate
Unconjugated

Applications


Application Notes
WB: 1 - 5 μg/ml; Sandwich ELISA: 10 μg/ml;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
PCF11; PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae); PCF11, cleavage and polyadenylation factor II subunit, homolog (S. cerevisiae); pre-mRNA cleavage complex 2 protein Pcf11; KIAA0824; PCF11p homolog
Entrez Gene ID
UniProt ID

Product Background


Antigen Description
Component of pre-mRNA cleavage complex II.
Pathway
Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Processing of Capped Intronless Pre-mRNA, organism-specific biosystem; Processing of Intronless Pre-mRNAs, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem.

Citations


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References


Zhang, ZQ; Gilmour, DS; et al. Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript. MOLECULAR CELL 21:65-74(2006).
Amrani, N; Minet, M; et al. PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I. MOLECULAR AND CELLULAR BIOLOGY 17:1102-1109(1997).

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