Rabbit Anti-Human PAX9 (a.a. 271-341) Polyclonal antibody

Name: Rabbit Anti-Human PAX9 (a.a. 271-341) Polyclonal antibody
SKU: DPABH-00583
Rating: 5 (1 reviews)

Rabbit Anti-Human PAX9 (a.a. 271-341) Polyclonal antibody for WB, IHC-P, IHC-F, IF

COA

SDS

Datasheet

Key features and details

Target: PAX9
Host: Rabbit
Application: WB, IHC-P, IHC-F, IF
Species Reactivity: Human, Mouse, Rat

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

KLH conjugated synthetic peptide derived from human PAX9: 271-341/341

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-2000
IHC-P: 1:100-500
IHC-F: 1:100-500
IF: 1:100-500

General Notes

This product (Catalog No. DPABH-00583) is a rabbit-derived IgG polyclonal antibody raised against human PAX9, targeting the amino acid region 271–341 of the protein. This antibody exhibits cross-reactivity with PAX9 from mouse and rat. It has been affinity purified by Protein A. This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. Mice lacking this gene exhibit impaired development of organs, musculature and the skeleton, including absent and abnormally developed teeth, and neonatal lethality. Mutations in the human gene are associated with selective tooth agenesis-3.
DPABH-00583 is suitable for use in Western blot (WB) (1:500–1:2,000), IHC-P (1:100–1:500), IHC-F (1:100–1:500), IF (immunofluorescence) (1:100–1:500) applications.
DPABH-00583 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

Paired box 9; Paired box gene 9; Paired box homeotic gene 9; Paired box protein 9; Paired box protein Pax 9; Paired box protein Pax9; Paired domain gene 9; PAX 9; PAX9_HUMAN

Entrez Gene ID

5083

UniProt ID

P55771

Citations

Have you cited DPABH-00583 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

WNT10A polymorphism may be a risk factor for non-syndromic hypodontia

GENETICS AND MOLECULAR RESEARCH

Authors: Zhang, S. J.; Wu, Z. Z.

Abstract

This study was designed to determine whether polymorphisms in the gene wingless-type MMTV integration site family, member 10A (WNT10A) are associated with non-syndromic hypodontia (tooth agenesis). A case-control study was performed involving 129 subjects with sporadic non-syndromic hypodontia (cases) and 218 healthy individuals (controls). DNA was obtained from whole blood and the ligase detection reaction method was used to analyze two single nucleotide polymorphisms (SNPs) of the WNT10A gene. A significant difference between cases and controls was observed in the allele and genotype frequencies of both SNPs (rs116998555 and rs147680216). For rs116998555, the presence of the T allele (the thymine variant) was associated with tooth agenesis [ odds ratio (OR) = 5.722; 95% confidence interval (CI) = 3.053-10.727; P < 0.001], while for rs147680216, the A allele (the adenine variant) correlated with this condition (OR = 2.665; 95% CI = 1.512-4.695; P < 0.001). We provide here the first case-control study evidence that risk of hypodontia may be related to the WNT10A polymorphism. Our results also confirm the importance of the Wnt pathway in tooth development.

Tooth agenesis in osteogenesis imperfecta related to mutations in the collagen type I genes

ORAL DISEASES

Authors: Malmgren, B.; Andersson, K.; Lindahl, K.; Kindmark, A.; Grigelioniene, G.; Zachariadis, V.; Dahllof, G.; Astrom, E.

Abstract

BackgroundOsteogenesis imperfecta (OI) is a heterogeneous group of disorders of connective tissue, mainly caused by mutations in the collagen type I genes (COL1A1 and COL1A2). Tooth agenesis is a common feature of OI. We investigated the association between tooth agenesis and collagen type I mutations in individuals with OI. Subjects and methodsIn this cohort study, 128 unrelated individuals with OI were included. Panoramic radiographs were analyzed regarding dentinogenesis imperfecta (DGI) and congenitally missing teeth. The collagen I genes were sequenced in all individuals, and in 25, multiplex ligation-dependent probe amplification was performed. ResultsMutations in the COL1A1 and COL1A2 genes were found in 104 of 128 individuals. Tooth agenesis was diagnosed in 17% (hypodontia 11%, oligodontia 6%) and was more frequent in those with DGI (P=0.016), and in those with OI type III, 47%, compared to those with OI types I, 12% (P=0.003), and IV, 13% (P=0.017). Seventy-five percent of the individuals with oligodontia (6 missing teeth) had qualitative mutations, but there was no association with OI type, gender, or presence of DGI. ConclusionThe prevalence of tooth agenesis is high (17%) in individuals with OI, and OI caused by a qualitative collagen I mutation is associated with oligodontia.