Anti-PARN monoclonal antibody, clone 5E7

Name: Anti-PARN monoclonal antibody, clone 5E7
SKU: DCABH-6795
Rating: 5 (1 reviews)

Mouse anti-Human PARN monoclonal antibody for WB

COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG2b

Clone

5E7

Species Reactivity

Human

Immunogen

Recombinant fragment corresponding to Human PARN aa 1-266.Sequence: MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTP EERYQKLKKH SMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQS SSIDFLASQG FDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCP VTIPEDQKKF I

Conjugate

Unconjugated

Applications

Application Notes

WB: 1/2000;

Target

Alternative Names

PARN; poly(A)-specific ribonuclease; poly(A) specific ribonuclease (deadenylation nuclease); poly(A)-specific ribonuclease PARN; DAN; deadenylation nuclease; deadenylating nuclease; polyadenylate-specific ribonuclease; poly(A)-specific ribonuclease (deade

Entrez Gene ID

5073

UniProt ID

B3KN69

Product Background

Antigen Description

3-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3-end poly(A) tail and the 5-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.

Pathway

Activation of Genes by ATF4, organism-specific biosystem; Deadenylation of mRNA, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Destabilization of mRNA by KSRP, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Gene Expression, organism-specific biosystem.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

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References

A nonradioactive assay for poly(A)-specific ribonuclease activity by methylene blue colorimetry

PROTEIN AND PEPTIDE LETTERS

Authors: Cheng, YA; Liu, WF; Yan, YB; Zhou, HM

Abstract

A simple nonradioactive assay, which was based on the specific shift of the absorbance maximum of methylene blue induced by its intercalation into poly(A) molecules, was developed for poly(A)-specific ribonuclease (PARN). A good linear relationship was found between the absorbance at 662 run and the poly(A) concentration. The assay conditions, including the concentration of methylene blue, the incubation temperature and time, and the poly(A) concentration were evaluated and optimized.

Jovibarba globifera (L) J Parn (Crassulaceae, Sempervivoideae) in Bratislava (Slovakia): Notes on taxonomy, nomenclature, distribution, ecology and conservation status

BIOLOGIA

Authors: Letz, R

Abstract

Evaluation of the name Jovibarba hirta subsp. glabrescens (SABR.) HOLUB, which has been usually applied for the majority of the populations of the genus Jovibarba from Slovakia, shows that its basionym is based on plants from granite localities in Bratislava. The only recent locality of Jovibarba in Bratislava is on the rock slopes of the Bratislava Castle hill, which represents an important refuge for the native flora just in the centre of the city. The population from this locality belongs to the variant intermediate between J. globifera subsp, globifera and J. globifera subsp. hirta, which also occurs on other localities in the lower altitudes in Slovakia (e.g. Male Karpaty Mts), the Czech Republic (Pavlovske vrchy Hills) and NE Austria. The occurrence of such intermediate variants supports a wide species concept within the genus Jovibarba, where J. hirta and J. globifera (syn. J. sobolifera) are treated as two subspecies within J. globifera. These intermediate populations are provisionally classified as J. globifera, without indication of subspecific name. J. globifera belongs to the category V (vulnerable) in the Red list of Bratislava.